Review



cd47 anti apoptosis function  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Novus Biologicals cd47 anti apoptosis function
    a <t>CD47</t> expression levels in benign prostatic hyperplasia, primary, and metastatic tumors analyzed from three published prostate cancer datasets – . b Biochemical recurrence (BCR)-free survival of the prostate cancer patients in the TCGA cohort. c Western blotting analysis of CD47 expression levels in three human prostate cancer cell lines. d A representative example of flow cytometry analysis of THCs in human primary macrophage and C4-2 cell co-culture model. Some CD86 + macrophages acquire Tag-it-Violet (TiV) dyes through engulfing cancer cells in co-culture. The cells maintained EGFP expression in this TiV + /CD86 + population were further identified as THCs. e Percentage of THCs in different co-culture models at different time points. f , g Percentage of THCs in 3-day co-cultures of U937 macrophages and CD47 overexpressed ( e ) or knockdown ( g ) cells. h – j Flow cytometry histograms and the corresponding violin plots showing BCL-2 expression in U937 macrophages and C4-2 ( h ), 22Rv1 ( i ), or DU145 ( j ) cells co-culture model. k Left: schematic diagram of proximity-ligation assay (PLA). Right: PLA signals of C4-2 cells with or without SIRPα stimulation. The dots represent mean values of total PLA counts per cell ( n = 7 images/group, 180 cells analyzed). l – n Effects of C4-2 cells unstimulated or stimulated by TNFα or TNFα combined with SIRPα. Capillary Western immunoassay (WES) showing BCL-2 and Lamin A/C expression ( l ), violin plots showing Annexin V expression ( m ), and bar graph showing cell viability ( n each dot represents the mean for each independent experiment). o Schematic diagram showing strong “don’t-eat-me” signals effectively inhibit phagocytosis. p Schematic diagram showing low “don’t-eat-me” signals allow macrophages to engulf cancer cells but partially increase the pro-survival function of cancer cells to escape from complete phagocytosis, subsequently resulting in THC formation. Data are the mean ± SD. P -values were determined using a two-sided unpaired t -test ( a , c , e – l , n ), a log-rank test ( b ), and a one-way ANOVA followed by Tukey test ( m ). Three independent experiments were carried out in ( c , e – g , l , n ). Source data for c , e – n are provided as a Source Data file.
    Cd47 Anti Apoptosis Function, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd47+anti+apoptosis+function/CD47+Antibody/pmc10582093-355-1-12
    Average 93 stars, based on 6 article reviews
    cd47 anti apoptosis function - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination"

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination

    Journal: Nature Communications

    doi: 10.1038/s41467-023-42303-5

    a CD47 expression levels in benign prostatic hyperplasia, primary, and metastatic tumors analyzed from three published prostate cancer datasets – . b Biochemical recurrence (BCR)-free survival of the prostate cancer patients in the TCGA cohort. c Western blotting analysis of CD47 expression levels in three human prostate cancer cell lines. d A representative example of flow cytometry analysis of THCs in human primary macrophage and C4-2 cell co-culture model. Some CD86 + macrophages acquire Tag-it-Violet (TiV) dyes through engulfing cancer cells in co-culture. The cells maintained EGFP expression in this TiV + /CD86 + population were further identified as THCs. e Percentage of THCs in different co-culture models at different time points. f , g Percentage of THCs in 3-day co-cultures of U937 macrophages and CD47 overexpressed ( e ) or knockdown ( g ) cells. h – j Flow cytometry histograms and the corresponding violin plots showing BCL-2 expression in U937 macrophages and C4-2 ( h ), 22Rv1 ( i ), or DU145 ( j ) cells co-culture model. k Left: schematic diagram of proximity-ligation assay (PLA). Right: PLA signals of C4-2 cells with or without SIRPα stimulation. The dots represent mean values of total PLA counts per cell ( n = 7 images/group, 180 cells analyzed). l – n Effects of C4-2 cells unstimulated or stimulated by TNFα or TNFα combined with SIRPα. Capillary Western immunoassay (WES) showing BCL-2 and Lamin A/C expression ( l ), violin plots showing Annexin V expression ( m ), and bar graph showing cell viability ( n each dot represents the mean for each independent experiment). o Schematic diagram showing strong “don’t-eat-me” signals effectively inhibit phagocytosis. p Schematic diagram showing low “don’t-eat-me” signals allow macrophages to engulf cancer cells but partially increase the pro-survival function of cancer cells to escape from complete phagocytosis, subsequently resulting in THC formation. Data are the mean ± SD. P -values were determined using a two-sided unpaired t -test ( a , c , e – l , n ), a log-rank test ( b ), and a one-way ANOVA followed by Tukey test ( m ). Three independent experiments were carried out in ( c , e – g , l , n ). Source data for c , e – n are provided as a Source Data file.
    Figure Legend Snippet: a CD47 expression levels in benign prostatic hyperplasia, primary, and metastatic tumors analyzed from three published prostate cancer datasets – . b Biochemical recurrence (BCR)-free survival of the prostate cancer patients in the TCGA cohort. c Western blotting analysis of CD47 expression levels in three human prostate cancer cell lines. d A representative example of flow cytometry analysis of THCs in human primary macrophage and C4-2 cell co-culture model. Some CD86 + macrophages acquire Tag-it-Violet (TiV) dyes through engulfing cancer cells in co-culture. The cells maintained EGFP expression in this TiV + /CD86 + population were further identified as THCs. e Percentage of THCs in different co-culture models at different time points. f , g Percentage of THCs in 3-day co-cultures of U937 macrophages and CD47 overexpressed ( e ) or knockdown ( g ) cells. h – j Flow cytometry histograms and the corresponding violin plots showing BCL-2 expression in U937 macrophages and C4-2 ( h ), 22Rv1 ( i ), or DU145 ( j ) cells co-culture model. k Left: schematic diagram of proximity-ligation assay (PLA). Right: PLA signals of C4-2 cells with or without SIRPα stimulation. The dots represent mean values of total PLA counts per cell ( n = 7 images/group, 180 cells analyzed). l – n Effects of C4-2 cells unstimulated or stimulated by TNFα or TNFα combined with SIRPα. Capillary Western immunoassay (WES) showing BCL-2 and Lamin A/C expression ( l ), violin plots showing Annexin V expression ( m ), and bar graph showing cell viability ( n each dot represents the mean for each independent experiment). o Schematic diagram showing strong “don’t-eat-me” signals effectively inhibit phagocytosis. p Schematic diagram showing low “don’t-eat-me” signals allow macrophages to engulf cancer cells but partially increase the pro-survival function of cancer cells to escape from complete phagocytosis, subsequently resulting in THC formation. Data are the mean ± SD. P -values were determined using a two-sided unpaired t -test ( a , c , e – l , n ), a log-rank test ( b ), and a one-way ANOVA followed by Tukey test ( m ). Three independent experiments were carried out in ( c , e – g , l , n ). Source data for c , e – n are provided as a Source Data file.

    Techniques Used: Expressing, Western Blot, Flow Cytometry, Co-Culture Assay, Knockdown, Proximity Ligation Assay

    Related Articles

    Expressing:

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
    Article Snippet: For CD47 anti-apoptosis function, cancer cells were treated with 20 ng/mL TNFα (Novus Biologicals, 210-TA/CF) or 20 ng/mL TNFα combined with 20 µg/mL SIRPα (R&D Systems, 9378-SA) for three days.. For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.

    Western Blot:

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
    Article Snippet: For CD47 anti-apoptosis function, cancer cells were treated with 20 ng/mL TNFα (Novus Biologicals, 210-TA/CF) or 20 ng/mL TNFα combined with 20 µg/mL SIRPα (R&D Systems, 9378-SA) for three days.. For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.

    Flow Cytometry:

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
    Article Snippet: For CD47 anti-apoptosis function, cancer cells were treated with 20 ng/mL TNFα (Novus Biologicals, 210-TA/CF) or 20 ng/mL TNFα combined with 20 µg/mL SIRPα (R&D Systems, 9378-SA) for three days.. For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.

    Co-Culture Assay:

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
    Article Snippet: For CD47 anti-apoptosis function, cancer cells were treated with 20 ng/mL TNFα (Novus Biologicals, 210-TA/CF) or 20 ng/mL TNFα combined with 20 µg/mL SIRPα (R&D Systems, 9378-SA) for three days.. For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.

    Knockdown:

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
    Article Snippet: For CD47 anti-apoptosis function, cancer cells were treated with 20 ng/mL TNFα (Novus Biologicals, 210-TA/CF) or 20 ng/mL TNFα combined with 20 µg/mL SIRPα (R&D Systems, 9378-SA) for three days.. For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.

    Proximity Ligation Assay:

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
    Article Snippet: For CD47 anti-apoptosis function, cancer cells were treated with 20 ng/mL TNFα (Novus Biologicals, 210-TA/CF) or 20 ng/mL TNFα combined with 20 µg/mL SIRPα (R&D Systems, 9378-SA) for three days.. For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.For cell viability assay, 4000 cells were seeded in 96-well culture plates for treatments and lysed using CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7571) at the end of the experiments.



    Similar Products

    cd47 anti apoptosis function  (Novus Biologicals)
    93
    Novus Biologicals cd47 anti apoptosis function
    a <t>CD47</t> expression levels in benign prostatic hyperplasia, primary, and metastatic tumors analyzed from three published prostate cancer datasets – . b Biochemical recurrence (BCR)-free survival of the prostate cancer patients in the TCGA cohort. c Western blotting analysis of CD47 expression levels in three human prostate cancer cell lines. d A representative example of flow cytometry analysis of THCs in human primary macrophage and C4-2 cell co-culture model. Some CD86 + macrophages acquire Tag-it-Violet (TiV) dyes through engulfing cancer cells in co-culture. The cells maintained EGFP expression in this TiV + /CD86 + population were further identified as THCs. e Percentage of THCs in different co-culture models at different time points. f , g Percentage of THCs in 3-day co-cultures of U937 macrophages and CD47 overexpressed ( e ) or knockdown ( g ) cells. h – j Flow cytometry histograms and the corresponding violin plots showing BCL-2 expression in U937 macrophages and C4-2 ( h ), 22Rv1 ( i ), or DU145 ( j ) cells co-culture model. k Left: schematic diagram of proximity-ligation assay (PLA). Right: PLA signals of C4-2 cells with or without SIRPα stimulation. The dots represent mean values of total PLA counts per cell ( n = 7 images/group, 180 cells analyzed). l – n Effects of C4-2 cells unstimulated or stimulated by TNFα or TNFα combined with SIRPα. Capillary Western immunoassay (WES) showing BCL-2 and Lamin A/C expression ( l ), violin plots showing Annexin V expression ( m ), and bar graph showing cell viability ( n each dot represents the mean for each independent experiment). o Schematic diagram showing strong “don’t-eat-me” signals effectively inhibit phagocytosis. p Schematic diagram showing low “don’t-eat-me” signals allow macrophages to engulf cancer cells but partially increase the pro-survival function of cancer cells to escape from complete phagocytosis, subsequently resulting in THC formation. Data are the mean ± SD. P -values were determined using a two-sided unpaired t -test ( a , c , e – l , n ), a log-rank test ( b ), and a one-way ANOVA followed by Tukey test ( m ). Three independent experiments were carried out in ( c , e – g , l , n ). Source data for c , e – n are provided as a Source Data file.
    Cd47 Anti Apoptosis Function, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd47+anti+apoptosis+function/CD47+Antibody/pmc10582093-355-1-12
    Average 93 stars, based on 1 article reviews
    cd47 anti apoptosis function - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a CD47 expression levels in benign prostatic hyperplasia, primary, and metastatic tumors analyzed from three published prostate cancer datasets – . b Biochemical recurrence (BCR)-free survival of the prostate cancer patients in the TCGA cohort. c Western blotting analysis of CD47 expression levels in three human prostate cancer cell lines. d A representative example of flow cytometry analysis of THCs in human primary macrophage and C4-2 cell co-culture model. Some CD86 + macrophages acquire Tag-it-Violet (TiV) dyes through engulfing cancer cells in co-culture. The cells maintained EGFP expression in this TiV + /CD86 + population were further identified as THCs. e Percentage of THCs in different co-culture models at different time points. f , g Percentage of THCs in 3-day co-cultures of U937 macrophages and CD47 overexpressed ( e ) or knockdown ( g ) cells. h – j Flow cytometry histograms and the corresponding violin plots showing BCL-2 expression in U937 macrophages and C4-2 ( h ), 22Rv1 ( i ), or DU145 ( j ) cells co-culture model. k Left: schematic diagram of proximity-ligation assay (PLA). Right: PLA signals of C4-2 cells with or without SIRPα stimulation. The dots represent mean values of total PLA counts per cell ( n = 7 images/group, 180 cells analyzed). l – n Effects of C4-2 cells unstimulated or stimulated by TNFα or TNFα combined with SIRPα. Capillary Western immunoassay (WES) showing BCL-2 and Lamin A/C expression ( l ), violin plots showing Annexin V expression ( m ), and bar graph showing cell viability ( n each dot represents the mean for each independent experiment). o Schematic diagram showing strong “don’t-eat-me” signals effectively inhibit phagocytosis. p Schematic diagram showing low “don’t-eat-me” signals allow macrophages to engulf cancer cells but partially increase the pro-survival function of cancer cells to escape from complete phagocytosis, subsequently resulting in THC formation. Data are the mean ± SD. P -values were determined using a two-sided unpaired t -test ( a , c , e – l , n ), a log-rank test ( b ), and a one-way ANOVA followed by Tukey test ( m ). Three independent experiments were carried out in ( c , e – g , l , n ). Source data for c , e – n are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination

    doi: 10.1038/s41467-023-42303-5

    Figure Lengend Snippet: a CD47 expression levels in benign prostatic hyperplasia, primary, and metastatic tumors analyzed from three published prostate cancer datasets – . b Biochemical recurrence (BCR)-free survival of the prostate cancer patients in the TCGA cohort. c Western blotting analysis of CD47 expression levels in three human prostate cancer cell lines. d A representative example of flow cytometry analysis of THCs in human primary macrophage and C4-2 cell co-culture model. Some CD86 + macrophages acquire Tag-it-Violet (TiV) dyes through engulfing cancer cells in co-culture. The cells maintained EGFP expression in this TiV + /CD86 + population were further identified as THCs. e Percentage of THCs in different co-culture models at different time points. f , g Percentage of THCs in 3-day co-cultures of U937 macrophages and CD47 overexpressed ( e ) or knockdown ( g ) cells. h – j Flow cytometry histograms and the corresponding violin plots showing BCL-2 expression in U937 macrophages and C4-2 ( h ), 22Rv1 ( i ), or DU145 ( j ) cells co-culture model. k Left: schematic diagram of proximity-ligation assay (PLA). Right: PLA signals of C4-2 cells with or without SIRPα stimulation. The dots represent mean values of total PLA counts per cell ( n = 7 images/group, 180 cells analyzed). l – n Effects of C4-2 cells unstimulated or stimulated by TNFα or TNFα combined with SIRPα. Capillary Western immunoassay (WES) showing BCL-2 and Lamin A/C expression ( l ), violin plots showing Annexin V expression ( m ), and bar graph showing cell viability ( n each dot represents the mean for each independent experiment). o Schematic diagram showing strong “don’t-eat-me” signals effectively inhibit phagocytosis. p Schematic diagram showing low “don’t-eat-me” signals allow macrophages to engulf cancer cells but partially increase the pro-survival function of cancer cells to escape from complete phagocytosis, subsequently resulting in THC formation. Data are the mean ± SD. P -values were determined using a two-sided unpaired t -test ( a , c , e – l , n ), a log-rank test ( b ), and a one-way ANOVA followed by Tukey test ( m ). Three independent experiments were carried out in ( c , e – g , l , n ). Source data for c , e – n are provided as a Source Data file.

    Article Snippet: For CD47 anti-apoptosis function, cancer cells were treated with 20 ng/mL TNFα (Novus Biologicals, 210-TA/CF) or 20 ng/mL TNFα combined with 20 μg/mL SIRPα (R&D Systems, 9378-SA) for three days.

    Techniques: Expressing, Western Blot, Flow Cytometry, Co-Culture Assay, Knockdown, Proximity Ligation Assay